Hulliger L, Sorkin E
Immunology. 1965 Oct;9(4):391-401.
The rate of antibody synthesis as measured by C-amino acid incorporation was estimated in thoracic duct lymph, buffy coat and spleen cells from rabbits hyperimmunized and boosted with human serum albumin (HSA). Cells from thoracic duct and buffy coat synthesized antibody in most experiments in small amounts, in two experiments in larger amounts. Spleen cells gave the highest values but also showed considerable variation from animal to animal. The comparison of antibody synthesis with the cellular composition of the different cell preparations led to the conclusion that the small percentage of pyroninophilic cells in lymph and buffy coat are responsible for the antibody synthesis and not the bulk of lymphocytes. Cell-bound antibody measured by fixation of radioactive HSA was found in spleen lymph and buffy coat cells. The major part of the detected cell-fixed antibody is presumably present on the surface of and within antibody-producing cells and not due to passively adsorbed cytophilic antibody on non-antibody-producing cells.
通过C -氨基酸掺入法测定抗体合成速率，对用人血清白蛋白（HSA）进行超免疫和加强免疫的兔的胸导管淋巴、血沉棕黄层和脾细胞进行了评估。在大多数实验中，胸导管和血沉棕黄层的细胞合成抗体的量较少，在两个实验中合成量较大。脾细胞合成抗体的值最高，但不同动物之间也显示出相当大的差异。将抗体合成与不同细胞制剂的细胞组成进行比较后得出结论，淋巴和血沉棕黄层中嗜派洛宁细胞的小比例负责抗体合成，而不是大部分淋巴细胞。通过放射性HSA的固定来测量的细胞结合抗体在脾淋巴和血沉棕黄层细胞中被发现。检测到的细胞固定抗体的主要部分可能存在于抗体产生细胞的表面和内部，而不是由于非抗体产生细胞上被动吸附的嗜细胞抗体。
