大肠杆菌转运RNA前体的体外核苷酸修饰
Nucleotide modification in vitro of the precursor of transfer RNA of Escherichia coli.
作者信息
Schaefer K P, Altman S, Söll D
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3626-30. doi: 10.1073/pnas.70.12.3626.
Abstract
Certain nucleotides in precursor RNA of tRNA(Tyr) of Escherichia coli were modified in vitro with a preparation of partially purified E. coli enzyme containing ribothymidine- and pseudouridine-forming activity. The only nucleotides modified in vitro are the same as those found modified in mature tRNA. The best substrate for these modifying enzymes is the RNase P cleavage product of the precursor RNA, which contains the mature tRNA sequence. Of the two pseudouridines found in mature tRNA, one (in the TPsiC sequence) can be formed in intact precursor RNA. The other (in the anticodon stem) can only be formed in the cleaved precursor RNA. The presence of modified nucleotides in the precursor RNA does not enhance its rate of cleavage by RNase P.
摘要
用含有形成核糖胸苷和假尿苷活性的部分纯化大肠杆菌酶制剂，在体外对大肠杆菌tRNA（Tyr）前体RNA中的某些核苷酸进行修饰。体外唯一被修饰的核苷酸与成熟tRNA中发现的修饰核苷酸相同。这些修饰酶的最佳底物是前体RNA的核糖核酸酶P切割产物，其包含成熟tRNA序列。在成熟tRNA中发现的两个假尿苷中，一个（在TPsiC序列中）可以在完整的前体RNA中形成。另一个（在反密码子茎中）只能在切割后的前体RNA中形成。前体RNA中修饰核苷酸的存在不会提高其被核糖核酸酶P切割的速率。
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