Grubman M J, Nakada D
J Bacteriol. 1974 Jan;117(1):227-31. doi: 10.1128/jb.117.1.227-231.1974.
In an MS2 phage ribonucleic acid (RNA)-directed in vitro protein-synthesizing system, the coat protein cistron and the adjacent RNA polymerase cistron are translated non-continuously. The ribosomes which have completed the synthesis of coat protein dissociate from the MS2 RNA and do not read through the intercistronic gap. Translation of the adjacent RNA polymerase cistron requires ribosomes other than those translating the coat protein cistron.
在一个MS2噬菌体核糖核酸(RNA)指导的体外蛋白质合成系统中,外壳蛋白顺反子和相邻的RNA聚合酶顺反子是不连续翻译的。完成外壳蛋白合成的核糖体从MS2 RNA上解离,不会通读顺反子间的间隔。相邻RNA聚合酶顺反子的翻译需要不同于翻译外壳蛋白顺反子的核糖体。