病毒信使核糖核酸的翻译：在小麦胚无细胞提取物中合成噬菌体Qβ蛋白
Translation of virus mRNA: synthesis of bacteriophage Q beta proteins in a cell-free extract from wheat embryo.
作者信息
Davies J W, Kaesberg P
出版信息
J Virol. 1973 Dec;12(6):1434-41. doi: 10.1128/JVI.12.6.1434-1441.1973.
Abstract
The RNA from bacteriophage Qbeta can be translated by cell-free extracts from wheat embryos. This translation, by 80S ribosomes, occurs at a low magnesium ion concentration. Three products are synthesized which coelectrophorese with Qbeta proteins synthesized in Escherichia coli extracts. The smallest of these has been identified as coat protein. Although the polycistronic bacteriophage message is translated with fidelity, the efficiency is much less than when the monocistronic brome mosaic virus coat protein message is translated.
摘要
来自噬菌体Qβ的RNA可被小麦胚无细胞提取物翻译。这种由80S核糖体进行的翻译在低镁离子浓度下发生。合成了三种产物，它们与在大肠杆菌提取物中合成的Qβ蛋白共电泳。其中最小的已被鉴定为外壳蛋白。尽管多顺反子噬菌体信息的翻译具有保真度，但其效率远低于单顺反子雀麦花叶病毒外壳蛋白信息的翻译效率。
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