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Translation of virus mRNA: synthesis of bacteriophage Q beta proteins in a cell-free extract from wheat embryo.病毒信使核糖核酸的翻译：在小麦胚无细胞提取物中合成噬菌体Qβ蛋白
J Virol. 1973 Dec;12(6):1434-41. doi: 10.1128/JVI.12.6.1434-1441.1973.
2
Translation of virus mRNA: synthesis of bacteriophage PP7 proteins in cell-free extracts from Pseudomonas aeruginosa.
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Translation of virus mRNA: protein synthesis directed by several virus RNAs in a cell-free extract from wheat germ.病毒信使核糖核酸的翻译：几种病毒核糖核酸在小麦胚芽无细胞提取物中指导的蛋白质合成。
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Translation of bacteriophage Q RNA by cytoplasmic extracts of mammalian cells.哺乳动物细胞胞质提取物对噬菌体Q RNA的翻译。
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Translation of bacteriophage Q messenger RNA in a murine Krebs 2 ascites tumor cell-free system.噬菌体Q信使核糖核酸在小鼠克雷布斯2腹水无细胞体系中的翻译
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Virology. 1974 Sep;61(1):281-6. doi: 10.1016/0042-6822(74)90262-1.
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Non-continuous translation of coat protein and ribonucleic acid polymerase cistrons in MS2 bacteriophage ribonucleic acid.MS2噬菌体核糖核酸中衣壳蛋白和顺反子核糖核酸聚合酶的非连续翻译。
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Ann Microbiol (Paris). 1976 Jan;127A(1):131-42.

引用本文的文献

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In vitro synthesis of turnip yellow mosaic virus coat protein in a wheat germ cell-free system.芜菁黄花叶病毒外壳蛋白在小麦胚无细胞体系中的体外合成
J Virol. 1976 Oct;20(1):196-202. doi: 10.1128/JVI.20.1.196-202.1976.
2
In Vitro Synthesis of Wheat (Triticum aestivum L.) Storage Proteins.小麦（Triticum aestivum L.）贮藏蛋白的体外合成
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3
Protein Synthesis and Accumulation in Bean Cotyledons during Growth.豆科植物子叶在生长过程中的蛋白质合成和积累。
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Cell-free Synthesis of the Major Storage Protein of the Bean, Phaseolus vulgaris L.无细胞合成菜豆主要贮藏蛋白
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Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterization.菜豆种子G1蛋白的信使核糖核酸：无细胞翻译及产物特性分析
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3196-200. doi: 10.1073/pnas.75.7.3196.
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Purification and translation of zein messenger RNA from maize endosperm protein bodies.从玉米胚乳蛋白体中纯化和翻译玉米醇溶蛋白信使核糖核酸
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7
Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ.源自市售小麦胚芽的高效蛋白质合成系统的特性分析
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Binding of wheat germ ribosomes to fragmented viral mRNA.小麦胚芽核糖体与片段化病毒信使核糖核酸的结合。
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9
In vitro translation of the three bacteriophage phi 6 RNAs.三种噬菌体phi 6 RNA的体外翻译
J Virol. 1980 Jul;35(1):249-51. doi: 10.1128/JVI.35.1.249-251.1980.
10
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.原核生物、真核生物和细胞器中蛋白质合成起始的比较。
Microbiol Rev. 1983 Mar;47(1):1-45. doi: 10.1128/mr.47.1.1-45.1983.

本文引用的文献

1
Purification and properties of bacteriophage MS2 and of its ribonucleic acid.噬菌体MS2及其核糖核酸的纯化与性质
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2
Mass isolation of viable wheat embryos.活性小麦胚的大规模分离
Nature. 1957 Jan 19;179(4551):160-1. doi: 10.1038/179160b0.
3
Cell-free protein synthesis programmed with R17 RNA: identification of two phage proteins.用R17 RNA编程的无细胞蛋白质合成：两种噬菌体蛋白的鉴定。
J Mol Biol. 1966 Oct 28;21(1):173-93. doi: 10.1016/0022-2836(66)90086-6.
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The amino acid sequence of the Q coat protein.Q外壳蛋白的氨基酸序列。
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Acrylamide gel electrophoresis of bacteriophage Q beta: electrophoresis of the intact virions and of the viral proteins.噬菌体Qβ的丙烯酰胺凝胶电泳：完整病毒粒子及病毒蛋白的电泳
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Natural read-through at the UGA termination signal of Q-beta coat protein cistron.Qβ外壳蛋白顺反子UGA终止信号处的天然通读。
Nat New Biol. 1971 Sep 15;234(50):206-9. doi: 10.1038/newbio234206a0.
7
Polysome formation with tobacco mosaic virus RNA.烟草花叶病毒RNA的多核糖体形成。
Proc Natl Acad Sci U S A. 1968 Apr;59(4):1243-50. doi: 10.1073/pnas.59.4.1243.
8
Translation of in vitro synthesized reovirus messenger RNAs into proteins of the size of reovirus capsid proteins in a mouse L cell extract.在小鼠L细胞提取物中，将体外合成的呼肠孤病毒信使核糖核酸翻译成呼肠孤病毒衣壳蛋白大小的蛋白质。
Biochem Biophys Res Commun. 1972 Mar 10;46(5):1816-23. doi: 10.1016/0006-291x(72)90056-3.
9
Origin of the small component of brome mosaic virus RNA.雀麦花叶病毒RNA小片段的起源
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10
Synthesis of polypeptides directed by the RNA of phage Q beta.由噬菌体Qβ的RNA指导的多肽合成。
Virology. 1970 Oct;42(2):401-14. doi: 10.1016/0042-6822(70)90283-7.

病毒信使核糖核酸的翻译：在小麦胚无细胞提取物中合成噬菌体Qβ蛋白

Translation of virus mRNA: synthesis of bacteriophage Q beta proteins in a cell-free extract from wheat embryo.

作者信息

Davies J W, Kaesberg P

出版信息

J Virol. 1973 Dec;12(6):1434-41. doi: 10.1128/JVI.12.6.1434-1441.1973.

DOI:10.1128/JVI.12.6.1434-1441.1973
PMID:4586778
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC356785/
Abstract

The RNA from bacteriophage Qbeta can be translated by cell-free extracts from wheat embryos. This translation, by 80S ribosomes, occurs at a low magnesium ion concentration. Three products are synthesized which coelectrophorese with Qbeta proteins synthesized in Escherichia coli extracts. The smallest of these has been identified as coat protein. Although the polycistronic bacteriophage message is translated with fidelity, the efficiency is much less than when the monocistronic brome mosaic virus coat protein message is translated.

摘要

来自噬菌体Qβ的RNA可被小麦胚无细胞提取物翻译。这种由80S核糖体进行的翻译在低镁离子浓度下发生。合成了三种产物，它们与在大肠杆菌提取物中合成的Qβ蛋白共电泳。其中最小的已被鉴定为外壳蛋白。尽管多顺反子噬菌体信息的翻译具有保真度，但其效率远低于单顺反子雀麦花叶病毒外壳蛋白信息的翻译效率。