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大肠杆菌噬菌体T1介导的半乳糖苷酶阳性(Gal+)转导。3. 受体细胞中转录和翻译的要求。

Transduction of Gal+ by coliphage T1. 3. Requirement for transcription and translation in recipient cells.

作者信息

Drexler H

出版信息

J Virol. 1973 Nov;12(5):1072-7. doi: 10.1128/JVI.12.5.1072-1077.1973.

Abstract

A 10- to 15-min derepression of a lambda prophage in a Gal(-) recipient during early infection with a transducing lysate of coliphage T1am will cause an increase in the efficiency of transduction of Gal(+). An increase in the efficiency of transduction occurs when the donor is either nonlysogenic or lysogenic for lambda; the increase is blocked by rifampin or chloramphenicol. With strain R901 it has been shown that efficient transduction can be blocked by treatment with rifampin after all chloramphenicol-sensitive steps have occurred.

摘要

在用大肠杆菌噬菌体T1am的转导裂解物进行早期感染期间,在Gal(-)受体中使λ原噬菌体去阻遏10至15分钟,会导致Gal(+)转导效率增加。当供体为非溶原性或对λ为溶原性时,转导效率都会增加;这种增加会被利福平或氯霉素阻断。对于R901菌株,已经表明,在所有氯霉素敏感步骤发生后,用利福平处理可阻断高效转导。

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Transduction by bacteriophage T1.噬菌体T1的转导作用
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1083-8. doi: 10.1073/pnas.66.4.1083.
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Interaction of rifamycin with bacterial RNA polymerase.利福霉素与细菌RNA聚合酶的相互作用。
Proc Natl Acad Sci U S A. 1968 Oct;61(2):667-73. doi: 10.1073/pnas.61.2.667.
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Genetic control of transcription during development of phage gamma.噬菌体γ发育过程中转录的遗传控制
Proc Natl Acad Sci U S A. 1967 Aug;58(2):576-83. doi: 10.1073/pnas.58.2.576.

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