Drexler H
J Virol. 1972 Feb;9(2):280-5. doi: 10.1128/JVI.9.2.280-285.1972.
One of the crossovers leading to the transduction of Escherichia coli W3350 or one of its derivatives for the Gal(+) marker by T1 grown on donors lysogenic for lambda must occur to the right of the galactose operon. The location of this crossover determined both the rate of transduction and the lambda genes which control the transduction by (lambda-gal)T1. When the crossover occurred either to the left of gene N or the right of the cI gene, it was affected in a positive fashion by the gene product of the N(+) gene. When the crossover occurred either to the left of gene N or between N and gene Q, its efficiency was not significantly affected by the expression of Q(+). However, crossovers to the right of gene Q were greatly stimulated by the presence of a functioning Q gene on the chromosome of (lambda-gal)T1 hybrid. The repressor made by the cI(+) gene of lambda blocked efficient transduction. Therefore, the control of efficient transduction by (lambda-gal)T1 is the same as the control of transcription of lambda. It is concluded that the increased efficiency of transduction by (lambda-gal)T1 in nonimmune recipients is not caused by any particular product of a gene but rather by the process of transcription itself.
在以λ溶原性供体菌上生长的T1介导下，导致大肠杆菌W3350或其衍生物之一转导Gal(+)标记的交叉事件之一必定发生在半乳糖操纵子的右侧。这个交叉事件的位置决定了转导速率以及控制（λ - gal）T1转导的λ基因。当交叉事件发生在基因N左侧或cI基因右侧时，它受到N(+)基因产物的正向影响。当交叉事件发生在基因N左侧或N与基因Q之间时，其效率不受Q(+)表达的显著影响。然而，在（λ - gal）T1杂种染色体上存在功能正常的Q基因时，发生在基因Q右侧的交叉事件会受到极大刺激。由λ的cI(+)基因产生的阻遏物会阻断高效转导。因此，（λ - gal）T1对高效转导的控制与λ转录的控制相同。得出的结论是，（λ - gal）T1在非免疫受体中转导效率的提高不是由基因的任何特定产物引起的，而是由转录过程本身导致的。
