Gordon M, Fraser L R, Dandekar P V
Anat Rec. 1975 Jan;181(1):95-111. doi: 10.1002/ar.1091810108.
Fertilized and unfertilized rabbit ova exposed to ruthenium red with the zona intact or after mechanical removal, for the demonstration of mucopolysaccharides on the surface coat. Ova were also exposed to Concanavalin A. Ruthenium red bound strongly to elements of the zona and increased the opacity of the plasmalemma. There was no notable change in staining of the vitellus following fertilization. Although there were ruthenium red stained bodies resembling cortical granules in crypts in the plasmalemma, there was no evidence for cortical granule extrusion of mucopolysaccharides. The Concanavalin reaction was seen over both unfertilized and fertilized ova, demonstrating receptors for terminal alpha-D-mannopyranosyl, alpha-D-glycopyranosyl, beta-D-fructofuranyosyl residues. The deposit on the fertilized egg surface was enormously enhanced indicating a change in the distribution of receptor sites.
完整透明带或经机械去除透明带后，将受精和未受精的兔卵暴露于钌红中，以显示表面被膜上的粘多糖。卵也暴露于刀豆球蛋白A。钌红与透明带成分强烈结合，并增加了质膜的不透明度。受精后卵黄的染色没有明显变化。虽然在质膜隐窝中有类似皮质颗粒的钌红染色小体，但没有证据表明粘多糖从皮质颗粒中挤出。在未受精和受精的卵上都观察到刀豆球蛋白反应，证明存在对末端α-D-甘露吡喃糖基、α-D-葡糖吡喃糖基、β-D-呋喃果糖基残基的受体。受精卵表面的沉积物大大增加，表明受体位点的分布发生了变化。
