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用伴刀豆球蛋白A和麦胚凝集素处理的兔卵子受精的电子显微镜评估

Electron microscope assessment of fertilization of rabbit ova treated with concanavalin A and wheat germ agglutinin.

作者信息

Gordon M, Dandekar P V

出版信息

J Exp Zool. 1976 Dec;198(3):437-42. doi: 10.1002/jez.1401980319.

DOI:10.1002/jez.1401980319
PMID:1036748
Abstract

Zonaless rabbit ova, exposed to Concanavalin A or Wheat Germ Agglutinin, then to uterine capacitated sperm produce pronuclear, 2 and 4 stage embryos that are indistinguishable from controls. Absence of cortical granules indicates that the ova were fertilized and not merely activated. Survival of lectin-bearing receptors during the period necessary for fertilization was evaluated in ova marked with ferritin-conjugated lectin.

摘要

无透明带兔卵,先暴露于伴刀豆球蛋白A或小麦胚凝集素,再与子宫获能精子接触,可产生原核、2细胞和4细胞期胚胎,这些胚胎与对照组无差异。皮质颗粒的缺失表明卵子已受精而非仅仅被激活。在用铁蛋白偶联凝集素标记的卵子中,评估了受精所需期间携带凝集素受体的存活情况。

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1
Electron microscope assessment of fertilization of rabbit ova treated with concanavalin A and wheat germ agglutinin.用伴刀豆球蛋白A和麦胚凝集素处理的兔卵子受精的电子显微镜评估
J Exp Zool. 1976 Dec;198(3):437-42. doi: 10.1002/jez.1401980319.
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Effects of wheat germ agglutinin on fertilization of mouse ova in vivo and in vitro.
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The effect of ruthenium red and Concanavalin A on the vitelline surface of fertilized and unfertilized rabbit ova.钌红和伴刀豆球蛋白A对受精和未受精兔卵卵黄膜表面的作用。
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