变形虫分离细胞表面的化学特性

Chemical characterization of the isolated cell surface of Amoeba.

作者信息

Allen H J, Ault C, Winzler R J, Danielli J F

出版信息

J Cell Biol. 1974 Jan;60(1):26-38. doi: 10.1083/jcb.60.1.26.

DOI:10.1083/jcb.60.1.26

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2109129/

Abstract

The cell surface has been isolated from uninucleate, freshwater, phagocytic amoebae by a new procedure. Several criteria were employed to demonstrate purity of the cell surface fraction. All morphological components of the tripartite surface were present in the isolated surface and the weight of the isolated surface was quantitatively accounted for by the components analyzed. Chemical analyses showed the presence of lipid, protein, and carbohydrate. Mannose was the predominant neutral sugar. Analyses for three different strains of Amoeba were similar. Phosphate was found to be the major anionic group in the cell surface material. Sulfate, uronic acid, sialic acid, muramic acid, and nonamidated glutamic acid and aspartic acid were absent. Evidence is presented suggesting that the phosphate is associated with an unidentified nonreducing polyol.

摘要

通过一种新方法从单核淡水吞噬性变形虫中分离出了细胞表面。采用了几个标准来证明细胞表面部分的纯度。三方表面的所有形态成分都存在于分离出的表面中，并且通过分析的成分对分离出的表面重量进行了定量计算。化学分析表明存在脂质、蛋白质和碳水化合物。甘露糖是主要的中性糖。对三种不同菌株的变形虫的分析结果相似。发现磷酸盐是细胞表面物质中的主要阴离子基团。不存在硫酸盐、糖醛酸、唾液酸、胞壁酸以及未酰胺化的谷氨酸和天冬氨酸。有证据表明磷酸盐与一种未鉴定的非还原性多元醇有关。

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。

J Biol Chem. 1951 Nov;193(1):265-75.

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ISOLATION AND PROPERTIES OF THE CELL SURFACE MEMBRANE OF AMOEBA PROTEUS.变形虫细胞膜的分离与特性

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Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.营养不良性肌肉的生物化学。线粒体琥珀酸 - 四氮唑还原酶和三磷酸腺苷酶。

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