Boklage C E, Wong E C, Bode V C
Genetics. 1973 Oct;75(2):221-30. doi: 10.1093/genetics/75.2.221.
Amber mutants previously mapped at seven sites in head gene F exhibit two contrasting phenotypes. Mutant F423, located in the right half of gene F, makes defective head particles with normal size lambdaDNA. These particles are complemented by crude lysates of mutants in other head genes. Mutants F785, F471 and F730 map in three adjacent sites in the left half of gene F. They are not complemented by lysates of other head mutants and do not produce headlike particles. Their lysates complement the incomplete heads present in F423. In vivo all three mutants are complemented by F423 but not by each other.
先前定位于头部基因F中七个位点的琥珀突变体表现出两种截然不同的表型。位于基因F右半部分的突变体F423产生具有正常大小λDNA的有缺陷的头部颗粒。这些颗粒可被其他头部基因的突变体粗裂解物互补。突变体F785、F471和F730定位于基因F左半部分的三个相邻位点。它们不能被其他头部突变体的裂解物互补,也不产生类头部颗粒。它们的裂解物可互补F423中存在的不完全头部。在体内,所有这三个突变体都可被F423互补,但彼此之间不能互补。