• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌修复合成缺陷的X射线敏感突变体的遗传和分子特征

Genetic and molecular characteristics of X-ray-sensitive mutants of Escherichia coli defective in repair synthesis.

作者信息

Kato T, Kondo S

出版信息

J Bacteriol. 1970 Nov;104(2):871-81. doi: 10.1128/jb.104.2.871-881.1970.

DOI:10.1128/jb.104.2.871-881.1970
PMID:4923076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC285071/
Abstract

Alleles responsible for X-ray-sensitive characteristics of three mutants of Escherichia coli B, which were also sensitive to ultraviolet (UV) irradiation, were mapped near metE locus, and named res-1, res-2, and res-3. All the res(-) mutants showed no host cell reactivability (Hcr(-)) for transducing deoxyribonucleic acid (DNA) of P1 phage irradiated by UV but they were Hcr(+) for infective DNA of P1 phage. Furthermore, they showed no detectable activity of DNA polymerase. Characteristics of allele res-1 were studied in detail. The mutant res-1 uvr(+) showed an extensive degradation of DNA after UV irradiation. Double mutants carrying res-1 uvrA(-), res-1 uvrB(-), and res-1 uvrC(-) showed no marked increase in UV sensitivity beyond that of the uvr(-) single mutants and only negligible UV-induced DNA degradation. The uvr(-) mutations showed no such suppressive effect on DNA degradation induced by X rays in these double mutants. It is concluded that res(-) mutants are defective in the second step (repair synthesis) of the excision repair process and that DNA polymerase is partly responsible for the assumed resynthesis step.

摘要

大肠杆菌B的三个突变体，它们对X射线敏感，同时也对紫外线（UV）照射敏感，负责这些特性的等位基因被定位在metE基因座附近，并分别命名为res - 1、res - 2和res - 3。所有res（-）突变体对经紫外线照射的P1噬菌体转导脱氧核糖核酸（DNA）均无宿主细胞修复活性（Hcr（-）），但对P1噬菌体的感染性DNA则具有Hcr（+）活性。此外，它们未表现出可检测到的DNA聚合酶活性。对res - 1等位基因的特性进行了详细研究。突变体res - 1 uvr（+）在紫外线照射后显示出DNA的广泛降解。携带res - 1 uvrA（-）、res - 1 uvrB（-）和res - 1 uvrC（-）的双突变体在紫外线敏感性方面相较于uvr（-）单突变体并无显著增加，且紫外线诱导的DNA降解可忽略不计。在这些双突变体中，uvr（-）突变对X射线诱导的DNA降解没有这种抑制作用。结论是，res（-）突变体在切除修复过程的第二步（修复合成）中存在缺陷，并且DNA聚合酶部分负责假定的再合成步骤。

相似文献

1
Genetic and molecular characteristics of X-ray-sensitive mutants of Escherichia coli defective in repair synthesis.大肠杆菌修复合成缺陷的X射线敏感突变体的遗传和分子特征
J Bacteriol. 1970 Nov;104(2):871-81. doi: 10.1128/jb.104.2.871-881.1970.
2
Excision repair characteristics of recB - res - and uvrC - strains of Escherichia coli.大肠杆菌recB - res - 和uvrC - 菌株的切除修复特性
J Bacteriol. 1972 Dec;112(3):1237-46. doi: 10.1128/jb.112.3.1237-1246.1972.
3
Ultraviolet- and X-ray-induced responses of a deoxyribonucleic acid polymerase-deficient mutant of Escherichia coli.紫外线和X射线诱导的大肠杆菌脱氧核糖核酸聚合酶缺陷型突变体的反应
J Bacteriol. 1971 Jul;107(1):61-7. doi: 10.1128/jb.107.1.61-67.1971.
4
The isolation and characterization of an X-ray-sensitive ultraviolet-resistant mutant of Escherichia coli.大肠杆菌X射线敏感紫外线抗性突变体的分离与鉴定。
Biochim Biophys Acta. 1971 Nov 29;254(1):144-54. doi: 10.1016/0005-2787(71)90121-3.
5
Repair of radiation-induced damage in Escherichia coli. II. Effect of rec and uvr mutations on radiosensitivity, and repair of x-ray-induced single-strand breaks in deoxyribonucleic acid.大肠杆菌中辐射诱导损伤的修复。II. rec和uvr突变对辐射敏感性的影响，以及脱氧核糖核酸中X射线诱导的单链断裂的修复
J Bacteriol. 1970 Jul;103(1):49-54. doi: 10.1128/jb.103.1.49-54.1970.
6
Requirement for protein synthesis in rec-dependent repair of deoxyribonucleic acid in Escherichia coli after ultraviolet or X irradiation.紫外线或X射线照射后大肠杆菌中依赖rec的脱氧核糖核酸修复过程中蛋白质合成的需求
J Bacteriol. 1972 Aug;111(2):575-85. doi: 10.1128/jb.111.2.575-585.1972.
7
Temperature-sensitive recovery of a mutant of Escherichia coli K-12 irradiated with ultraviolet light.紫外线照射的大肠杆菌K-12突变体的温度敏感型恢复
J Bacteriol. 1971 Sep;107(3):623-32. doi: 10.1128/jb.107.3.623-632.1971.
8
Properties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene.基因30（脱氧核糖核酸连接酶）和rII基因缺陷的噬菌体T4突变体的特性
J Virol. 1971 Feb;7(2):260-6. doi: 10.1128/JVI.7.2.260-266.1971.
9
Isolation and characterization of an Escherichia coli ruv mutant which forms nonseptate filaments after low doses of ultraviolet light irradiation.一株大肠杆菌ruv突变体的分离与鉴定，该突变体在低剂量紫外线照射后形成无隔膜细丝。
J Bacteriol. 1974 Feb;117(2):337-44. doi: 10.1128/jb.117.2.337-344.1974.
10
Fate of thymine-containing dimers in the deoxyribonucleic acid of ultraviolet-irradiated mutator T1 Escherichia coli transductants.紫外线照射的诱变型T1大肠杆菌转导子脱氧核糖核酸中含胸腺嘧啶二聚体的命运
Mutat Res. 1976 Jan;34(1):55-68. doi: 10.1016/0027-5107(76)90261-x.

引用本文的文献

1
Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.构建一个能过量表达大肠杆菌DNA聚合酶I的大蛋白水解片段（克列诺片段）的质粒。
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1830-4. doi: 10.1073/pnas.80.7.1830.
2
DNA polymerases in prokaryotes and eukaryotes: mode of action and biological implications.原核生物和真核生物中的DNA聚合酶：作用模式及生物学意义
Experientia. 1983 Jan 15;39(1):1-25. doi: 10.1007/BF01960616.
3
Detection of the catalytic activities of DNA polymerases and their associated exonucleases following SDS-polyacrylamide gel electrophoresis.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后DNA聚合酶及其相关核酸外切酶催化活性的检测。
Nucleic Acids Res. 1981 Apr 24;9(8):1825-39. doi: 10.1093/nar/9.8.1825.
4
Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.大肠杆菌xth突变体中X射线诱导的脱氧核糖核酸单链断裂的修复
J Bacteriol. 1980 Mar;141(3):1424-7. doi: 10.1128/jb.141.3.1424-1427.1980.
5
Alkaline sucrose gradient sedimentation of chromosomal deoxyribonucleic acid from Escherichia coli PolA + and PolA - strains during thymine starvation.胸腺嘧啶饥饿期间大肠杆菌PolA+和PolA-菌株染色体脱氧核糖核酸的碱性蔗糖梯度沉降
J Bacteriol. 1971 Dec;108(3):1422-3. doi: 10.1128/jb.108.3.1422-1423.1971.
6
Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.DNA聚合酶I缺陷型大肠杆菌突变体中新复制DNA链的缓慢连接。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2954-7. doi: 10.1073/pnas.68.12.2954.
7
Influence of growth phase on UV induced lethality and DNA breakdown in a Kornberg polymerase deficient resA strain of Escherichia coli.生长阶段对大肠杆菌Kornberg聚合酶缺陷型resA菌株紫外线诱导的致死率和DNA断裂的影响。
Mol Gen Genet. 1971;112(2):110-6. doi: 10.1007/BF00267489.
8
Ultraviolet- and X-ray-induced responses of a deoxyribonucleic acid polymerase-deficient mutant of Escherichia coli.紫外线和X射线诱导的大肠杆菌脱氧核糖核酸聚合酶缺陷型突变体的反应
J Bacteriol. 1971 Jul;107(1):61-7. doi: 10.1128/jb.107.1.61-67.1971.
9
Auxotroph accumulation in deoxyribonucleic acid polymeraseless strains of Escherichia coli K-12.大肠杆菌K-12无脱氧核糖核酸聚合酶菌株中营养缺陷型的积累
J Bacteriol. 1971 Jun;106(3):797-801. doi: 10.1128/jb.106.3.797-801.1971.
10
Studies on the ultraviolet-sensitive mutants of blue-green alga Synechocystis aquatilis Sanv.水生集胞藻Sanv.的蓝藻紫外线敏感突变体研究
Arch Mikrobiol. 1972;86(4):349-60. doi: 10.1007/BF00424991.

本文引用的文献

1
REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES.异亮氨酸和缬氨酸生物合成中的调控机制。II. 两个操纵基因的鉴定
J Bacteriol. 1965 Mar;89(3):654-60. doi: 10.1128/jb.89.3.654-660.1965.
2
EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA.细菌中紫外线损伤DNA修复复制的证据
J Mol Biol. 1964 Aug;9:395-410. doi: 10.1016/s0022-2836(64)80216-3.
3
PHOTOREVERSAL AND "DARK REPAIR" OF MUTATIONS TO PROTOTROPHY INDUCED BY ULTRAVIOLET LIGHT IN PHOTOREACTIVABLE AND NON-PHOTOREACTIVABLE STRAINS OF ESCHERICHIA COLI.紫外线诱导的大肠杆菌光可活化和非光可活化菌株中向原养型突变的光逆转和“暗修复”
Mutat Res. 1964 May;106:22-36. doi: 10.1016/0027-5107(64)90049-1.
4
RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12.大肠杆菌K-12中DNA上紫外线诱导胸腺嘧啶二聚体的释放
Proc Natl Acad Sci U S A. 1964 Feb;51(2):293-300. doi: 10.1073/pnas.51.2.293.
5
THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM.胸腺嘧啶二聚体从DNA中的消失：一种纠错机制。
Proc Natl Acad Sci U S A. 1964 Feb;51(2):226-31. doi: 10.1073/pnas.51.2.226.
6
ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XV. PURIFICATION AND PROPERTIES OF A POLYMERASE FROM BACILLUS SUBTILIS.脱氧核糖核酸的酶促合成。十五。枯草芽孢杆菌中一种聚合酶的纯化及性质
J Biol Chem. 1964 Jan;239:259-68.
7
ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.脱氧核糖核酸的酶促合成。十四。大肠杆菌脱氧核糖核酸聚合酶的进一步纯化及性质
J Biol Chem. 1964 Jan;239:222-32.
8
An unusual DNA extracted from bacteria infected with phage T2.从被噬菌体T2感染的细菌中提取出的一种异常DNA。
Proc Natl Acad Sci U S A. 1963 Mar 15;49(3):366-72. doi: 10.1073/pnas.49.3.366.
9
A method for selecting radiation-sensitive mutants of Escherichia coli.一种筛选大肠杆菌辐射敏感突变体的方法。
Genetics. 1962 Sep;47(9):1219-24. doi: 10.1093/genetics/47.9.1219.
10
A genetic locus in E. coli K12 that controls the reactivation of UV-photoproducts associated with thymine in DNA.大肠杆菌K12中一个控制与DNA中胸腺嘧啶相关的紫外线光产物再活化的基因座。
Proc Natl Acad Sci U S A. 1962 Dec 15;48(12):2109-15. doi: 10.1073/pnas.48.12.2109.