Jobson A, McLaughlin M, Cook F D, Westlake D W
Appl Microbiol. 1974 Jan;27(1):166-71. doi: 10.1128/am.27.1.166-171.1974.
Replicate field plots comprising a control, plus oil, plus oil and bacteria, plus oil and fertilizer (urea-phosphate; 27:27:0), and plus oil, bacteria, and fertilizer were monitored over a 308-day period for changes in bacterial and mold numbers. Changes in the chemical composition of the oil applied to the plots was followed by using chromatographic techniques. Application of fertilizer resulted in a stimulation of bacterial numbers and in the rate of utilization of n-alkane components of the saturate fraction. The application of oil-utilizing bacteria, however, resulted in only a slightly accelerated rate of utilization of n-alkane components of chain lengths C20 to C25. The isoprenoids, phytane and pristane, were still present in gas-liquid chromatography profiles after digestion of the n-alkane components of the saturate fraction. Those plots which received fertilizer showed an accelerated rate of recovery of native vegetation.
对包括对照、加石油、加石油和细菌、加石油和肥料(磷酸脲;27:27:0)以及加石油、细菌和肥料的重复田间小区进行了为期308天的监测,以观察细菌和霉菌数量的变化。通过色谱技术跟踪施用于小区的石油化学成分的变化。施肥导致细菌数量增加以及饱和馏分中正构烷烃成分的利用速率加快。然而,施用利用石油的细菌仅使碳链长度为C20至C25的正构烷烃成分的利用速率略有加快。在饱和馏分的正构烷烃成分消化后,类异戊二烯、植烷和姥鲛烷仍存在于气液色谱图中。那些施肥的小区显示出原生植被恢复速率加快。