Jobson A, Cook F D, Westlake D W
Appl Microbiol. 1972 Jun;23(6):1082-9. doi: 10.1128/am.23.6.1082-1089.1972.
The utilization of two crude oil samples of different quality at 4 and 30 C has been studied by using pure and mixed bacterial cultures obtained by enrichment procedures. Growth, emulsification, and utilization occurred readily at both temperatures. The crude oil residue is increased in specific gravity and readily sediments out of solution. A comparison of the chemical analysis of the oils by liquid and gas-liquid chromatographic procedures before and after growth showed that the n-saturate fraction had been preferentially used. Some utilization of the aromatic fraction also occurred. Enrichments obtained with a high-quality crude oil were not as effective in utilizing a lower quality crude oil as sole carbon source as a population enriched on the low-quality crude oil.
利用富集培养法获得的纯细菌培养物和混合细菌培养物,研究了两种不同质量的原油样品在4℃和30℃下的利用情况。在这两个温度下,细菌都能迅速生长、乳化并利用原油。原油残渣的比重增加,很容易从溶液中沉淀出来。通过液相色谱和气相色谱法对生长前后的原油进行化学分析比较,结果表明正构饱和烃馏分被优先利用。芳烃馏分也有一定程度的利用。以高质量原油进行富集培养得到的菌群,在利用低质量原油作为唯一碳源时,不如以低质量原油富集得到的菌群有效。