多头绒泡菌的黏变形体的无菌培养
Axenic culture of myxamoebae of the myxomycete Physarum polycephalum.
作者信息
Goodman E M
出版信息
J Bacteriol. 1972 Jul;111(1):242-7. doi: 10.1128/jb.111.1.242-247.1972.
Abstract
Myxamoebae of the acellular slime mold Physarum polycephalum have been cultured axenically in a soluble medium. The growth medium contains bovine serum albumin, embryo extracts, liver infusion broth, peptone, and glucose. Cell densities ranging from 3 x 10(6) to 5 x 10(6) cells/ml have been obtained with this medium. To date, myxamoebae have been serially transferred more than 100 times without deleterious effect.
摘要
多核变形虫黏菌多头绒泡菌的黏变形体已在可溶性培养基中进行无菌培养。生长培养基包含牛血清白蛋白、胚胎提取物、肝脏浸出液、蛋白胨和葡萄糖。使用这种培养基已获得了3×10⁶至5×10⁶个细胞/毫升的细胞密度。迄今为止，黏变形体已连续传代100多次而没有产生有害影响。
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