Bernstam V A, Gray R H, Bernstein I A
Arch Microbiol. 1980 Nov;128(1):34-40. doi: 10.1007/BF00422302.
The effects of the microtubule-disrupting drugs, colchicine, vinblastine, podophyllotoxin, griseofulvin, and lumicolchicine (10(-5) M), on protein and RNA synthesis were studied in Physarum polycephalum amoebae in culture. All, except lumicolchicine, were found to simultaneously reduce the rate of protein synthesis and stimulate RNA synthesis. These results parallel the effects seen in cells exposed to heat shock. Treatment of cells with a microfilament-disrupting drug, cytochalasin B (10 micrograms/ml in ethanol), resulted in a reduced rate of protein synthesis after 2 h compared to a similar effect by vinblastine in 5--15 min. A morphological abnormality, microtubule paracrystals, were seen associated with centrioles in vinblastine-treated cells in which protein synthesis had been reduced by 50%. Vinblastine and podophyllotoxin were shown to interfere with the recovery of protein synthesis after inhibition by low or elevated temperatures. The possible role of microtubules in regulating the translational response of a cell to an external environmental stimulus is discussed.
研究了微管破坏药物秋水仙碱、长春碱、鬼臼毒素、灰黄霉素和光秋水仙碱（10⁻⁵ M）对多头绒泡菌培养的变形虫中蛋白质和RNA合成的影响。发现除光秋水仙碱外，所有药物均同时降低蛋白质合成速率并刺激RNA合成。这些结果与热休克处理的细胞中观察到的效应相似。用微丝破坏药物细胞松弛素B（乙醇中10微克/毫升）处理细胞，2小时后蛋白质合成速率降低，而长春碱在5 - 15分钟内产生类似效果。在长春碱处理的细胞中，蛋白质合成降低了50%，观察到与中心粒相关的形态异常——微管副晶体。已表明长春碱和鬼臼毒素会干扰低温或高温抑制后蛋白质合成的恢复。讨论了微管在调节细胞对外部环境刺激的翻译反应中的可能作用。
