Sriurairatna S, Bhamarapravati N, Phalavadhtana O
Infect Immun. 1973 Dec;8(6):1017-28. doi: 10.1128/iai.8.6.1017-1028.1973.
In dengue type-2 virus-infected neurones of suckling mice, formation of single-membrane vesicles is observed in the distended cisternae of the endoplasmic reticulum mostly of the perinuclear zone around 72 h after inoculation. Electron-dense 50-nm virus particles are arranged in chains in these distended cisternae; some form small crystalloid aggregates. Aberrant particles of different shapes are also seen in the distended cisternae about the same time that the virus particles appear. Parallel filamentous structures are occasionally observed in the cisternae that contain very few virions, either characteristic or aberrant. Increasing cytopathic changes are present after 75 to 96 h. There is an intense vesicular formation. Large numbers of virions and aberrant particles are seen either in the endoplasmic reticulum cisternae or smooth membrane vesicles. They are spread throughout the neurocytoplasm, extending into the dendrites. Dengue virions which are enclosed in fairly intact membrane-bound vesicles are released during cytolysis of the neurones. Morphogenesis of dengue virus type 2 is discussed.
在接种后约72小时，在乳鼠的登革2型病毒感染神经元中，在内质网（主要是核周区的内质网）扩张的潴泡中观察到单膜囊泡的形成。电子致密的50纳米病毒颗粒在这些扩张的潴泡中呈链状排列；一些形成小的晶体聚集体。在病毒颗粒出现的大约同一时间，在扩张的潴泡中也可见到不同形状的异常颗粒。在含有极少病毒粒子（无论是典型的还是异常的）的潴泡中偶尔观察到平行丝状结构。75至96小时后出现越来越多的细胞病变变化。有强烈的囊泡形成。在内质网潴泡或光滑膜囊泡中可见大量病毒粒子和异常颗粒。它们遍布神经细胞质，延伸至树突。包裹在相当完整的膜结合囊泡中的登革病毒粒子在神经元细胞溶解过程中释放。讨论了登革2型病毒的形态发生。
