Li Shufen, Shi Yongxia, Zheng Kui, Dai Jun, Li Xiaobo, Yuan Shuai, Chen Ling, Huang Jicheng
Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, P. R. China.
State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, P. R. China.
PLoS One. 2017 Jan 17;12(1):e0169256. doi: 10.1371/journal.pone.0169256. eCollection 2017.
The recent outbreaks of Zika virus (ZIKV) disease have caused worldwide concerns. Guangdong province is one of the commercial centers in China and communicates frequently with the epidemic areas. To date, 65.2% of the ZIKV infection cases in China were imported via port of entry in Guangdong. The continuous surveillance of imported cases is crucial for the prevention and control of potential ZIKV infection outbreak in China. In this study, a strain of ZIKV was isolated from the serum of a 6-year-old child returning from Venezuela. The morphology of the ZIKV was analyzed in vivo and in vitro by electron microscopy, and clusters of virus particles were found in the loose cytoplasmic membrane structures. The genomic sequence of the isolated ZIKV was determined, and the alignment and phylogenetic analysis identified one unique amino acid substitution occurring in the non-structural protein 4B (NS4B), and the isolated virus belonged to the Asian lineage.
近期寨卡病毒（ZIKV）病的爆发引起了全球关注。广东省是中国的商业中心之一，与疫区频繁往来。截至目前，中国65.2%的寨卡病毒感染病例是通过广东口岸输入的。持续监测输入病例对于预防和控制中国潜在的寨卡病毒感染爆发至关重要。在本研究中，从一名从委内瑞拉返回的6岁儿童血清中分离出一株寨卡病毒。通过电子显微镜在体内和体外分析了寨卡病毒的形态，在疏松的细胞质膜结构中发现了病毒颗粒簇。测定了分离出的寨卡病毒的基因组序列，比对和系统发育分析确定在非结构蛋白4B（NS4B）中发生了一个独特的氨基酸替换，且分离出的病毒属于亚洲谱系。
