Sternheimer R
JAMA. 1975 Feb 24;231(8):826-32.
A mixture of aqueous solutions of National fast blue, a copper-phthalocyanine dye, and pyronin B, a red xanthene dye, when added to fresh urinary sediment, supravitally stains benign or malignant cells and the various types of casts and their inclusions. The stain facilitates identification of the formed elements and particularly aids in the differentiation of polymorphonuclear leukocytes from lymphocytes, histiocytes, plasma cells, and renal tubular cells. A variable staining of casts and their inclusions has been observed. Tumor cells may be recognized by nuclear abnormalities or, in case of hyperchromatic tendency, by a very rapid and early uptake of dye preceding that of the surrounding cells. The staining method is rapid and simple enough for routine urinalysis and screening procedures.
将铜酞菁染料直接耐晒蓝和红色呫吨染料派若宁B的水溶液混合物添加到新鲜尿沉渣中时，可对良性或恶性细胞以及各种类型的管型及其内含物进行活体内染色。该染色有助于识别尿沉渣中的有形成分，尤其有助于区分多形核白细胞与淋巴细胞、组织细胞、浆细胞及肾小管细胞。观察到管型及其内含物有不同程度的染色。肿瘤细胞可通过核异常识别，或者在有染色质增多倾向的情况下，通过比周围细胞更快且更早摄取染料来识别。该染色方法快速简便，适用于常规尿液分析和筛查程序。
