纸色谱法作为鉴定厌氧菌的辅助手段。
Paper chromatography as an adjunct in the identification of anaerobic bacteria.
作者信息
Slifkin M, Hercher H J
出版信息
Appl Microbiol. 1974 Mar;27(3):500-5. doi: 10.1128/am.27.3.500-505.1974.
Abstract
Modified paper chromatography procedures for the analysis of fatty acids produced by anaerobic bacteria are described. Both ethylamine and hydroxylamine derivatives of fatty acids were prepared from inoculated anaerobic culture broth. The derivatives were spotted on chromatography paper and developed with appropriate solvents. Paper chromatography is a valuable alternative to gas liquid chromatography as an ancillary procedure in the identification of anaerobic bacteria in the clinical bacteriology laboratory.
摘要
本文描述了用于分析厌氧菌产生的脂肪酸的改良纸色谱法。脂肪酸的乙胺衍生物和羟胺衍生物均由接种的厌氧培养基肉汤制备。将衍生物点在色谱纸上，并用适当的溶剂展开。在临床细菌学实验室中，作为鉴定厌氧菌的辅助方法，纸色谱法是气相色谱法的一种有价值的替代方法。
相似文献
1
Paper chromatography as an adjunct in the identification of anaerobic bacteria.纸色谱法作为鉴定厌氧菌的辅助手段。
Appl Microbiol. 1974 Mar;27(3):500-5. doi: 10.1128/am.27.3.500-505.1974.
2
Gas-liquid chromatography of short-chain fatty acids on Dexsil 300 GC.在Dexsil 300 GC上对短链脂肪酸进行气液色谱分析。
J Chromatogr. 1972 Dec 20;74(2):335-8. doi: 10.1016/s0021-9673(01)86164-1.
3
Modification of the gas-liquid chromatography procedure and evaluation of a new column packing material for the identification of anaerobic bacteria.气液色谱法程序的改进及一种用于厌氧细菌鉴定的新型柱填充材料的评估
J Clin Microbiol. 1975 Jul;2(1):1-7. doi: 10.1128/jcm.2.1.1-7.1975.
4
Ultrasensitive detection of certain microbial metabolites by gas chromatography.气相色谱法对某些微生物代谢产物的超灵敏检测
Anal Biochem. 1967 Sep;20(3):548-50. doi: 10.1016/0003-2697(67)90300-4.
5
Volatile fatty acids in normal human physiological fluids.
Clin Chim Acta. 1970 Sep;29(3):369-74. doi: 10.1016/0009-8981(70)90004-5.
6
Gas chromatographic separation and determination of micro quantities of C1-C7 branched and straight-chain saturated fatty acids.
J Chromatogr. 1969 Feb 25;39(4):398-406. doi: 10.1016/s0021-9673(01)98028-8.
7
Volatile fatty acids in contents of pendulous crops and normal croups of turkey hens.
Poult Sci. 1973 Mar;52(2):510-2. doi: 10.3382/ps.0520510.
8
Ascaris lumbricoides: succinate and tiglate in hemolymph.
Exp Parasitol. 1971 Apr;29(2):208-14. doi: 10.1016/0014-4894(71)90026-9.
9
Simplified gas chromatographic procedure for identification of bacterial metabolic products.用于鉴定细菌代谢产物的简化气相色谱法。
Appl Microbiol. 1973 Feb;25(2):287-9. doi: 10.1128/am.25.2.287-289.1973.
10
End products of bovine spermatozoan metabolism as influenced by incubation diluent and temperature.
J Dairy Sci. 1967 Apr;50(4):558-61. doi: 10.3168/jds.S0022-0302(67)87465-4.
引用本文的文献
1
Quantitative gas chromatography of Bacteroides species under different growth conditions.不同生长条件下拟杆菌属的定量气相色谱分析。
Antonie Van Leeuwenhoek. 1978;44(1):1-14. doi: 10.1007/BF00400072.
本文引用的文献
1
Separation of saturated mono-hydroxamic acids by partition chromatography on paper.通过纸上分配色谱法分离饱和单异羟肟酸。
Aust J Sci Res B. 1951 May;4(2):180-6. doi: 10.1071/bi9510180.
2
Use of paper partition chromatography in the identification of the volatile fatty acids.纸分配色谱法在挥发性脂肪酸鉴定中的应用。
Nature. 1950 Sep 23;166(4221):522. doi: 10.1038/166522a0.
3
DETECTION OF VOLATILE FATTY ACIDS PRODUCED BY OBLIGATE GRAM-NEGATIVE ANAEROBES.专性革兰氏阴性厌氧菌产生的挥发性脂肪酸的检测
J Med Lab Technol. 1963 Oct;20:266-8.
4
[Paper chromatography of volatile aliphatic acids of C1 to C6; use in determination of anaerobic bacteria].
Ann Inst Pasteur Lille. 1956;8:13-52.
5
[Study of aliphatic volatile acids from C1 to C6 produced by 215 strains of anaerobic bacteria].[215株厌氧菌产生的C1至C6脂肪族挥发性酸的研究]
Ann Inst Pasteur (Paris). 1956 Feb;90(2):229-33.
6
Bacterial identification: clues from computers and chemistry.
Can J Med Technol. 1972 Jan-Feb;34(1):2-16.
7
Thin-layer chromatography of N,N-dimethyl-p-aminobenzolazophenacyl esters of volatile fatty acids and hydroxy acids.挥发性脂肪酸和羟基酸的N，N-二甲基对氨基苯偶氮苯甲酰酯的薄层色谱法
Anal Biochem. 1972 Mar;46(1):62-6. doi: 10.1016/0003-2697(72)90395-8.
8
Simplified gas chromatographic procedure for identification of bacterial metabolic products.用于鉴定细菌代谢产物的简化气相色谱法。
Appl Microbiol. 1973 Feb;25(2):287-9. doi: 10.1128/am.25.2.287-289.1973.
9
Anaerobic bacteriology in the clinical laboratory.临床实验室中的厌氧菌学
Am J Med Technol. 1972 Nov;38(11):441-6.
Zotero 插件