用于鉴定细菌代谢产物的简化气相色谱法。
Simplified gas chromatographic procedure for identification of bacterial metabolic products.
作者信息
Carlsson J
出版信息
Appl Microbiol. 1973 Feb;25(2):287-9. doi: 10.1128/am.25.2.287-289.1973.
Abstract
A rapid and simple procedure is described for analysis of fermentation products from anaerobic bacteria grown in glucose broth. A 1-ml sample of the culture is drained through cation-exchange resin in a Pasteur pipette. The effluent fluid is directly analyzed isothermally in a gas chromatograph for volatile fatty acids (C(2) to C(6)) as well as for lactic, pyruvic, and succinic acids. This procedure is considered to be suitable for routine use in clinical bacteriology.
摘要
本文描述了一种快速简便的方法，用于分析在葡萄糖肉汤中生长的厌氧细菌的发酵产物。将1毫升培养物样品通过巴斯德吸管中的阳离子交换树脂排出。流出液直接在气相色谱仪中进行等温分析，以检测挥发性脂肪酸（C2至C6）以及乳酸、丙酮酸和琥珀酸。该方法被认为适用于临床细菌学的常规应用。
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本文引用的文献
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