用甲氧苄啶治愈R因子大肠杆菌。
Curing of an R factor Escherichia coli by trimethoprim.
作者信息
Pinney R J, Smith J T
出版信息
Antimicrob Agents Chemother. 1973 Jun;3(6):670-6. doi: 10.1128/AAC.3.6.670.
R factor 1818, which we have shown previously to be eliminated by thymine starvation, was cured from three strains of Escherichia coli K-12 by overnight exposure to trimethoprim. Elimination was abolished in the presence of added thymine or thymidine, which suggests that curing is the result of the induction of thymineless conditions by trimethoprim. Starvation of the required amino acids proline and histidine had little effect on elimination, whereas methionine deprivation enhanced it. R factor curing was abolished by the presence of chloramphenicol, and it is concluded that protein synthesis is required for elimination to occur. It is suggested that elimination may result from the activity of a nuclease which is synthesized or induced during both direct thymine starvation and by trimethoprim treatment.
R 因子 1818，我们之前已证明可通过胸腺嘧啶饥饿将其消除，通过将三株大肠杆菌 K - 12 过夜暴露于甲氧苄啶，该因子从这些菌株中被治愈。在添加胸腺嘧啶或胸苷的情况下，消除作用被消除，这表明治愈是甲氧苄啶诱导无胸腺嘧啶条件的结果。必需氨基酸脯氨酸和组氨酸的饥饿对消除作用影响很小，而蛋氨酸缺乏则增强了消除作用。氯霉素的存在使 R 因子治愈作用被消除，得出的结论是消除过程需要蛋白质合成。有人提出消除可能是由一种核酸酶的活性导致的，这种核酸酶在直接胸腺嘧啶饥饿和甲氧苄啶处理过程中都会合成或被诱导产生。
Zotero 插件