Crawford J J, Sconyers J R, Moriarty J D, King R C, West J F
Appl Microbiol. 1974 May;27(5):927-32. doi: 10.1128/am.27.5.927-932.1974.
Both prereduced molten agar and broth and aerobic molten agar and broth were inoculated with blood samples collected from patients with periodontitis, but in otherwise good health, both before and after extraction of two or more teeth. Postoperative blood samples from 23 of 25 patients sampled yielded anaerobic and facultative species. Colony counts from nine samples yielded from less than 1 to over 100 colonies per ml of blood. Organisms detected were species belonging to the genera Bacteroides, Fusobacterium, Peptostreptococcus, Leptotrichia, Propionibacterium, Peptococcus, Veillonella, plus Streptococcus mitis, S. salivarius, vibrio forms, and strains resembling S. mutans. The data indicate that prereduced anaerobically sterilized culture medium with polyanethol sulfonate is effective for detecting anaerobic species in bacteremia and that anaerobic species can be prevalent in bacteremias immediately after tooth extraction in patients with periodontitis.
将牙周炎患者(总体健康状况良好)在拔除两颗或更多牙齿前后采集的血样,接种到预先还原的熔化琼脂和肉汤以及需氧熔化琼脂和肉汤中。25例采样患者中有23例的术后血样培养出厌氧和兼性菌种。9份血样的菌落计数结果为每毫升血液中菌落数少于1个至超过100个。检测到的微生物包括拟杆菌属、梭杆菌属、消化链球菌属、纤毛菌属、丙酸杆菌属、消化球菌属、韦荣球菌属的菌种,以及缓症链球菌、唾液链球菌、弧菌形态的细菌,还有类似变形链球菌的菌株。数据表明,含聚茴香脑磺酸钠的预先还原厌氧灭菌培养基对于检测菌血症中的厌氧菌种有效,并且在牙周炎患者拔牙后立即发生的菌血症中厌氧菌种可能很普遍。