四种用于厌氧菌生长的市售布鲁氏菌琼脂培养基的比较。
Comparison of four commercial brucella agar media for growth of anaerobic organisms.
作者信息
Mangels J I, Douglas B P
机构信息
Clinical Microbiology Department, Good Samaritan Hospital, San Jose, California 95124.
出版信息
J Clin Microbiol. 1989 Oct;27(10):2268-71. doi: 10.1128/jcm.27.10.2268-2271.1989.
Four different commercial brucella blood agar plating media (Anaerobe Systems, BBL Microbiology Systems, Remel, and Scott Laboratories) were compared for the abilities to recover anaerobic organisms from clinical specimens and to support the growth of American Type Culture Collection anaerobic stock cultures. Following 24 h of incubation in an anaerobe chamber, Anaerobe Systems prereduced, anaerobically sterilized brucella plates yielded 63% of the total clinical anaerobe isolates, the Scott medium yielded 51%, the Remel medium yielded 42%, and the BBL medium yielded 37%. Poor growth of Peptostreptococcus magnus, P. anaerobius, Fusobacterium necrophorum, F. nucleatum, and pigmented Bacteroides spp. was observed on brucella media obtained from BBL, Remel, and Scott. Data obtained with stock anaerobic cultures showed that Anaerobe Systems plates yielded good growth and produced a larger colony size with all of the strains tested in 1 day, whereas poor growth of Peptostreptococcus spp., B. melaninogenicus, and Fusobacterium spp. was noted on brucella media from BBL, Remel, and Scott.
比较了四种不同的商业布鲁氏菌血琼脂平板培养基（厌氧系统公司、BBL微生物系统公司、Remel公司和斯科特实验室）从临床标本中分离厌氧生物以及支持美国典型培养物保藏中心厌氧储备培养物生长的能力。在厌氧培养箱中孵育24小时后，厌氧系统公司预先还原、厌氧灭菌的布鲁氏菌平板分离出的临床厌氧生物占总分离物的63%，斯科特培养基为51%，Remel培养基为42%，BBL培养基为37%。在从BBL、Remel和斯科特公司获得的布鲁氏菌培养基上，观察到巨大消化链球菌、厌氧消化链球菌、坏死梭杆菌、具核梭杆菌和产色素拟杆菌属生长不佳。用储备厌氧培养物获得的数据表明，厌氧系统公司的平板生长良好，在1天内对所有测试菌株都产生了更大的菌落，而在BBL、Remel和斯科特公司的布鲁氏菌培养基上，消化链球菌属、产黑色素拟杆菌和梭杆菌属生长不佳。
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