Herrlich P, Rahmsdorf H J, Pai S H, Schweigher M
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1088-92. doi: 10.1073/pnas.71.4.1088.
Phage T7 discontinues host gene expression by translational and transcriptional control mechanisms. Translational control is exerted by the T7 translational-repressor. This protein inhibits the synthesis of beta-galactosidase (EC 3.2.1.23) in vivo and in vitro and the synthesis of the T3 enzyme S-adenosylmethioninehydrolase (EC 3.3.1.-). The translational-repressor does not interfere with T7-specific enzyme synthesis. The T7 translational-repressor purifies with the initiation factors. The repressor interacts with the initiation of translation of host enzymes. The translational-repressor gene is close to the promotor for RNA polymerase of Escherichia coli.
T7噬菌体通过翻译和转录控制机制终止宿主基因表达。翻译控制由T7翻译阻遏物施加。这种蛋白质在体内和体外抑制β-半乳糖苷酶(EC 3.2.1.23)的合成以及T3酶S-腺苷甲硫氨酸水解酶(EC 3.3.1.-)的合成。翻译阻遏物不干扰T7特异性酶的合成。T7翻译阻遏物与起始因子一起纯化。阻遏物与宿主酶翻译的起始相互作用。翻译阻遏物基因靠近大肠杆菌RNA聚合酶的启动子。