噬菌体T7诱导的蛋白激酶对大肠杆菌依赖DNA的RNA聚合酶的体内和体外磷酸化作用。

In vivo and in vitro phosphorylation of DNA-dependent RNA polymerase of Escherichia coli by bacteriophage-T7-induced protein kinase.

作者信息

Zillig W, Fujiki H, Blum W, Janeković D, Schweiger M, Rahmsdorf H, Ponta H, Hirsch-Kauffmann M

出版信息

Proc Natl Acad Sci U S A. 1975 Jul;72(7):2506-10. doi: 10.1073/pnas.72.7.2506.

After infection with bacteriophage T7 the beta' and to a lesser extent the beta subunits of E. coli DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) are phosphorylated by a phage-gene-encoded protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37). The phosphorylation occurs on threonine residues and appears site-specific. It is probably the molecular basis of the early transcriptional control.