一种来自垂体生长激素的高血糖肽：用胃蛋白酶制备及在ob-ob小鼠中的测定
A hyperglycemic peptide from pituitary growth hormone: preparation with pepsin and assay in ob-ob mice.
作者信息
Lostroh A J, Krahl M E
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1244-6. doi: 10.1073/pnas.71.4.1244.
A fragment that induces glucose intolerance in hereditarily obese mice has been prepared from pituitary growth hormone(sheep and human) by controlled digestion with pepsin in 0.05 M sodium acetate buffer, pH 3.7. The digest was purified by column chromatography on Bio-Gel P-6, followed by chromatography on Bio-Rex 70 and Bio-Gel P-2; in each successive step peptide was quantitated with the fluorescamine procedure. The fragment from sheep growth hormone has these characteristics: (1) It induces glucose intolerance in fasted ob/ob female mice when injected subcutaneously in a divided dose 15 min before, and concurrently with, glucose. (2) It is a basie peptide, as judged from its elution behavior on Bio-Rex 70, H(+) form. (3) It does not crossreact with antiserum to human growth hormone in radioimmunoassay. Further purification and initial sequence determinations have been undertaken.
通过在pH 3.7的0.05M醋酸钠缓冲液中用胃蛋白酶进行可控消化，从垂体生长激素（绵羊和人类）中制备出了一种能在遗传性肥胖小鼠中诱发葡萄糖不耐受的片段。消化产物先用Bio-Gel P-6进行柱色谱纯化，然后再用Bio-Rex 70和Bio-Gel P-2进行色谱分离；在每一步后续操作中，都用荧光胺法对肽进行定量。绵羊生长激素片段具有以下特性：（1）在禁食的ob/ob雌性小鼠中，在注射葡萄糖前15分钟皮下分剂量注射该片段并同时注射葡萄糖时，会诱发葡萄糖不耐受。（2）从其在Bio-Rex 70（H(+)形式）上的洗脱行为判断，它是一种碱性肽。（3）在放射免疫测定中，它与抗人生长激素血清不发生交叉反应。已经进行了进一步的纯化和初步的序列测定。
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