Lehmkuhl H D, Frey M L
Appl Microbiol. 1974 Jun;27(6):1170-1. doi: 10.1128/am.27.6.1170-1171.1974.
Mycoplasma colonies were grown on microscope slides overlaid with solid medium. After the slides were dried, washed, acetone fixed, and stained with fluorescein-conjugated antisera, specific immunofluorescence was easily observed against a dark background.
支原体菌落生长在覆盖有固体培养基的显微镜载玻片上。载玻片干燥、洗涤、丙酮固定并用荧光素偶联抗血清染色后,在深色背景下很容易观察到特异性免疫荧光。