Payment P, Corbeil M, Chagnon A
Can J Microbiol. 1978 Jun;24(6):689-92. doi: 10.1139/m78-116.
Mycoplasma contaminants of animal and human cell cultures were rapidly detected and identified by an indirect immunofluorescent technique. Cells suspected of being contaminated by mycoplasmas were grown as monolayers on chamber slides in a culture medium selected to promote mycoplasmal growth. Before fixation by acetone, the monolayers were subjected to a hypotonic treatment to cause swelling of the mycoplasmas. Detection and identification were then performed by indirect immunofluorescence using rabbit antisera to various mycoplasma species. The correlation between results obtained by the standard isolation procedure and those obtained by this method was very close.
采用间接免疫荧光技术可快速检测和鉴定动物及人类细胞培养物中的支原体污染物。疑似被支原体污染的细胞在选定的促进支原体生长的培养基中，以单层形式生长在培养室载玻片上。在用丙酮固定之前，对单层细胞进行低渗处理以使支原体肿胀。然后使用针对各种支原体物种的兔抗血清，通过间接免疫荧光进行检测和鉴定。标准分离程序所得结果与本法所得结果之间的相关性非常密切。
