Hoppe I, Roth J
Genetics. 1974 Apr;76(4):633-54. doi: 10.1093/genetics/76.4.633.
Salmonella phage P22 has been used in the construction of three sorts of specialized transducing phage: P22 proAB, P22 proABlac and P22 argF. The bacterial genes carried are derived from E. coli K12. Since E. coli and Salmonella chromosomes recombine very poorly, E. coli genes cannot be transduced into Salmonella recipients by P22's generalized transduction mechanism. Therefore, stable inheritance of E. coli material provides a means of detecting specialized transduction. Formation of these phages was possible because the P22 prophage recognizes an attachment site in the E. coli F' prolac episome. Salmonella strains carrying the F' prolac episome can be lysogenized by P22 so as to leave the prophage inserted into the E. coli material of the F' factor. Improper prophage excision can then lead to formation of P22 specialized phages carrying E. coli genetic material.
沙门氏菌噬菌体P22已被用于构建三种类型的特异性转导噬菌体:P22 proAB、P22 proABlac和P22 argF。所携带的细菌基因源自大肠杆菌K12。由于大肠杆菌和沙门氏菌的染色体重组能力很差,大肠杆菌基因不能通过P22的普遍性转导机制转导到沙门氏菌受体中。因此,大肠杆菌物质的稳定遗传提供了一种检测特异性转导的方法。这些噬菌体的形成是可能的,因为P22原噬菌体识别大肠杆菌F' prolac附加体中的附着位点。携带F' prolac附加体的沙门氏菌菌株可以被P22溶源化,从而使原噬菌体插入F'因子的大肠杆菌物质中。然后,原噬菌体的不适当切除会导致携带大肠杆菌遗传物质的P22特异性噬菌体的形成。