携带沙门氏菌组氨酸操纵子的F-部分二倍体和φ80高频转导噬菌体的构建。
Derivation of an F-merogenote and a phi-80 high-frequency transducing phage carrying the histidine operon os Salmonella.
作者信息
Voll M J
出版信息
J Bacteriol. 1972 Feb;109(2):741-50. doi: 10.1128/jb.109.2.741-750.1972.
Abstract
An F' factor, FS400, carrying the his operon, the gnd gene, and the rfb gene cluster of Salmonella typhimurium was isolated. FS400 was introduced into an Escherichia coli strain having a lengthy deletion of the his gene region. From this strain, Hfr derivatives were isolated which had the F' factor integrated in the tonB locus near the attachment site of phi80. One of the Hfr strains was lysogenized with a heat-inducible, h mutant of phi80, and from this strain a high-frequency transducing phage carrying the his genes and the gnd gene of Salmonella was isolated.
摘要
分离出了携带鼠伤寒沙门氏菌组氨酸操纵子、gnd基因和rfb基因簇的F'因子FS400。将FS400导入到组氨酸基因区域存在长片段缺失的大肠杆菌菌株中。从该菌株中分离出Hfr衍生物，其F'因子整合在靠近phi80附着位点的tonB位点。其中一个Hfr菌株用phi80的热诱导h突变体进行溶原化，从该菌株中分离出携带沙门氏菌组氨酸基因和gnd基因的高频转导噬菌体。
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