Simchen G
Genetics. 1974 Apr;76(4):745-53. doi: 10.1093/genetics/76.4.745.
Sporulation of diploid yeasts (Saccharomyces cerevisiae), homozygous or heterozygous for temperature-sensitive mitotic cell-cycle mutations, was examined at the restrictive and permissive temperatures. Twenty genes, represented by 32 heterozygotes and 60 homozygotes, were divided into three groups, showing (i) normal sporulation, (ii) no sporulation at the restrictive temperature but normal sporulation at the permissive temperature, (iii) no sporulation at both temperatures. Group (i) as well as several other strains were tested for their meiotic behavior with regard to intragenic recombination and haploidization. The conclusion reached was that all the mitotic nuclear-division and DNA-synthesis functions were required in meiosis. The only cell-division mutations not to affect meiosis were in three cytokinesis loci and in one budemergence locus.
对携带温度敏感型有丝分裂细胞周期突变的纯合或杂合二倍体酵母（酿酒酵母）在限制温度和允许温度下的孢子形成进行了检测。由32个杂合子和60个纯合子代表的20个基因被分为三组，分别表现为：（i）正常孢子形成；（ii）在限制温度下不形成孢子，但在允许温度下正常形成孢子；（iii）在两个温度下均不形成孢子。对第（i）组以及其他几个菌株在基因内重组和单倍体化方面的减数分裂行为进行了测试。得出的结论是，减数分裂需要所有有丝分裂核分裂和DNA合成功能。唯一不影响减数分裂的细胞分裂突变存在于三个胞质分裂位点和一个芽出现位点。
