酿酒酵母中的基因重组与减数分裂的启动
Genetic recombination and commitment to meiosis in Saccharomyces.
作者信息
Esposito R E, Esposito M S
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3172-6. doi: 10.1073/pnas.71.8.3172.
Abstract
Diploid cells of the yeast Saccharomyces cerevisiae become committed to recombination at meiotic levels without becoming committed to the meiotic disjunction of chromosomes. These two events of the meiotic process can be separated by removing cells from a meiosis-inducing medium and returning them to a medium that promotes vegetative cell division. Cells removed at an appropriate time remain diploid, revert to mitosis, and display recombination with meiotic-like frequencies. Those removed after this time are committed to the completion of meiosis. Diploids of three conditional sporulation-deficient mutants (spo1-1, spo2-1, and spo3-1) have been examined for recombination at restrictive temperatures. All exhibit commitment to recombination without commitment to meiotic disjunction as in the wild type. Cells of spo1-1/spo1-1 do not replicate the spindle pole body for meiosis I; thus, recombination ability can be acquired by cells that do not proceed beyond this cytological stage.
摘要
酿酒酵母的二倍体细胞在减数分裂水平上开始进行重组，但并不进入染色体的减数分裂分离过程。减数分裂过程中的这两个事件可以通过将细胞从减数分裂诱导培养基中取出，然后再放回促进营养细胞分裂的培养基中而分开。在适当时间取出的细胞保持二倍体状态，恢复有丝分裂，并以类似减数分裂的频率进行重组。在此之后取出的细胞则进入减数分裂的完成阶段。已经对三个条件性孢子形成缺陷突变体（spo1-1、spo2-1和spo3-1）的二倍体细胞在限制温度下的重组情况进行了检测。所有这些突变体都像野生型一样，表现出在不进入减数分裂分离的情况下开始进行重组。spo1-1/spo1-1细胞在减数分裂I时不复制纺锤极体；因此，未超过这个细胞学阶段的细胞也能够获得重组能力。
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