Tait R C, Rodriguez R L, Boyer H W
Mol Gen Genet. 1977 Mar 16;151(3):327-31. doi: 10.1007/BF00268797.
Investigation of tetracycline resistance genetically determined by the plasmid pSC101 and several recombinants of pSC101 containing EcoRI generated DNA fragments inserted at the EcoRI site has revealed significant differences in the phenotypic expression of that resistance. The altered phenotypes of the recombinant plasmids may be the result of the location of the EcoRI site of pSC101, which has been determined to be near the genetic elements involved with tetracycline resistance.
对由质粒pSC101以及pSC101的几个重组体(这些重组体含有插入到EcoRI位点的EcoRI产生的DNA片段)遗传决定的四环素抗性进行的研究揭示了该抗性在表型表达上的显著差异。重组质粒改变的表型可能是pSC101的EcoRI位点位置的结果,该位点已被确定靠近与四环素抗性有关的遗传元件。