大肠杆菌K-12 F-Con突变体的特性分析
Characterization of an Escherichia coli K-12 F-Con-mutant.
作者信息
Havekes L M, Hoekstra W P
出版信息
J Bacteriol. 1976 May;126(2):593-600. doi: 10.1128/jb.126.2.593-600.1976.
Abstract
An Escherichia coli K-12 F-mutant defective in conjugation was isolated by means of a zygotic induction enrichment procedure. The recipient ability of the mutant was reduced about 50 times owing to a block in one of the first steps of the conjugation process. In the mutant, cell envelope alterations could not be observed. Sensitivity toward detergents, antibiotics, and phages was unaltered. The mutation appeared to be co-transducible with pyrD. The linkage order in the region of the mutation is origin KL 99-con-pyrD-aroA.
摘要
通过合子诱导富集程序分离出一株在接合过程中有缺陷的大肠杆菌K-12 F-突变体。由于接合过程第一步中的一个阻断，该突变体的受体能力降低了约50倍。在该突变体中，未观察到细胞包膜改变。对去污剂、抗生素和噬菌体的敏感性未改变。该突变似乎与pyrD共转导。突变区域的连锁顺序是原点KL 99-接合-pyrD-aroA。
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