大肠杆菌B-r稳态生长过程中的大分子组成
Macromolecular composition during steady-state growth of Escherichia coli B-r.
作者信息
Dennis P P, Bremer H
出版信息
J Bacteriol. 1974 Jul;119(1):270-81. doi: 10.1128/jb.119.1.270-281.1974.
Abstract
By using Escherichia coli B/r, the cellular amounts of ribonucleic acid (RNA) and protein were determined as a function of the steady-state growth rate (0.67 to 2.40 doublings per h) by a method which combines measurements of the RNA to deoxyribonucleic acid (DNA) ratio and the differential rate of ribosomal protein synthesis with the Cooper and Helmstetter theory of DNA replication. The results indicate that the ratios RNA/DNA, RNA/protein, and protein/DNA give linear relationships with the growth rate (above 1.2 doublings per h), whereas RNA/cell and protein/cell show a more complex growth rate dependency. The significance of these relationships is discussed. Finally, a detailed description of the growth parameters and composition of E. coli B/r is presented.
摘要
通过使用大肠杆菌B/r，采用一种将核糖核酸（RNA）与脱氧核糖核酸（DNA）的比率测量、核糖体蛋白合成的微分速率与库珀和赫尔姆斯泰特的DNA复制理论相结合的方法，测定了RNA和蛋白质的细胞含量与稳态生长速率（每小时0.67至2.40次倍增）之间的函数关系。结果表明，RNA/DNA、RNA/蛋白质和蛋白质/DNA的比率与生长速率（每小时超过1.2次倍增）呈线性关系，而RNA/细胞和蛋白质/细胞则表现出更复杂的生长速率依赖性。讨论了这些关系的意义。最后，给出了大肠杆菌B/r生长参数和组成的详细描述。
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