细胞上表面：其在培养中无法支持活跃的细胞运动。
The upper cell surface: its inability to support active cell movement in culture.
作者信息
DiPasquale A, Bell P B
出版信息
J Cell Biol. 1974 Jul;62(1):198-214. doi: 10.1083/jcb.62.1.198.
Abstract
A variety of epithelial cells and fibroblasts fail to move over one another's upper surfaces in culture, resulting in monolayering. The failure of seeded fibroblasts to adhere to and spread on epithelial cell surfaces suggests that monolayering in culture is due to the lack of adhesion of the upper cell surface, at least of epithelial cells. Seeded fibroblasts and postmitotic, rounded fibroblasts likewise fail to spread on the upper surfaces of spread fibroblasts, suggesting that the inability of the upper cell surface to support spreading may be a general phenomenon. Inert particles and cell processes do not adhere directly to the upper cell surface. However, they can initiate adhesions to the surface at a cell's free margin, suggesting a variation of adhesive properties over a cell's surface.
摘要
多种上皮细胞和成纤维细胞在培养时无法在彼此的上表面移动，从而形成单层。接种的成纤维细胞无法粘附并铺展在上皮细胞表面，这表明培养中的单层形成是由于上层细胞表面，至少是上皮细胞表面缺乏粘附性。接种的成纤维细胞和有丝分裂后的圆形成纤维细胞同样无法在铺展的成纤维细胞的上表面铺展，这表明上层细胞表面无法支持铺展可能是一种普遍现象。惰性颗粒和细胞突起不会直接粘附在上层细胞表面。然而，它们可以在细胞的自由边缘处引发与表面的粘附，这表明细胞表面的粘附特性存在差异。
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