Sanders E J
In Vitro. 1982 Jan;18(1):71-8. doi: 10.1007/BF02796387.
Previous work has shown that when chick embryo mesoderm tissue is seeded onto the free, dorsal surface of established sheets of embryonic epithelial endoblast, the former penetrates the latter and spreads on the underlying artificial substratum. In this work, the surface charge on the epithelial sheet has been altered, prior to seeding the mesoderm, to ascertain whether such a change could alter the behavior of the mesoderm with respect to the free surface of the epithelium. Charge alteration was accomplished using the polycations, poly-L-lysine. Surface charge characteristics were examined ultrastructurally using cationized and anionized ferritin. Results showed that although surface charge changes were detectable, there was no difference in the behavior of the mesoderm with respect to the endoblast. Neuraminidase did not detectably affect the epithelial surface charge. These results are consistent with the view that changes in substratum surface charge are not necessarily correlated with changes in adhesiveness.
先前的研究表明，当将鸡胚中胚层组织接种到已形成的胚胎上皮内胚层片的游离背表面上时，前者会穿透后者并在下面的人工基质上扩散。在这项研究中，在接种中胚层之前改变了上皮片的表面电荷，以确定这种变化是否会改变中胚层相对于上皮游离表面的行为。使用聚阳离子聚-L-赖氨酸实现电荷改变。使用阳离子化和阴离子化铁蛋白通过超微结构检查表面电荷特征。结果表明，尽管可检测到表面电荷变化，但中胚层相对于内胚层的行为没有差异。神经氨酸酶未对上皮表面电荷产生可检测到的影响。这些结果与以下观点一致，即基质表面电荷的变化不一定与粘附性的变化相关。
