DNA中脱嘌呤位点的体外修复
In vitro repair of apurinic sites in DNA.
作者信息
Verly W G, Gossard F, Crine P
出版信息
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2273-5. doi: 10.1073/pnas.71.6.2273.
Abstract
Apurinic sites disappear from DNA during an incubation of the DNA with the Escherichia coli endonuclease specific for apurinic sites, DNA polymerase I (EC 2.7.7.7.), and T4 ligase (EC 6.5.1.1). Omission of any one of these three enzymes and, in particular, omission of the endonuclease specific for apurinic sites prevents this in vitro repair.
摘要
在DNA与对脱嘌呤位点具有特异性的大肠杆菌内切核酸酶、DNA聚合酶I（EC 2.7.7.7.）以及T4连接酶（EC 6.5.1.1）一起温育的过程中，脱嘌呤位点会从DNA上消失。省略这三种酶中的任何一种，尤其是省略对脱嘌呤位点具有特异性的内切核酸酶，都会阻止这种体外修复。
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