阻遏蛋白结合位点在乳糖操纵子中的位置。
The location of the repressor binding sites in the lac operon.
作者信息
Reznikoff W S, Winter R B, Hurley C K
出版信息
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2314-8. doi: 10.1073/pnas.71.6.2314.
Abstract
The affinity of various mutant lac DNAs for the lac repressor was examined by means of a competition binding assay. The results indicate that there exist at least two binding sites for the lac repressor. The primary binding site is coincidental with the operator, as defined by genetic studies. Mutations of the promoter or the Z gene have little or no effect on the repressor binding properties of this primary binding site, thereby confirming that the operator is distinct from lacP and Z. The secondary binding site(s), which has a 25- to 31-fold lower affinity for the repressor, is located within the operator proximal third of the Z gene.
摘要
通过竞争结合试验检测了各种突变型乳糖操纵子DNA与乳糖阻遏物的亲和力。结果表明，乳糖阻遏物至少存在两个结合位点。主要结合位点与遗传学研究定义的操纵基因一致。启动子或Z基因的突变对该主要结合位点的阻遏物结合特性几乎没有影响，从而证实操纵基因与lacP和Z不同。对阻遏物亲和力低25至31倍的次要结合位点位于Z基因靠近操纵基因的三分之一区域内。
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