反密码子在甲硫氨酰 - tRNA合成酶与细菌起始tRNA相互作用中的作用。

The role of the anticodon in the interaction between methionyl-tRNA synthetase and bacterial initiator tRNA.

作者信息

Bruton C J, Clark B F

出版信息

Nucleic Acids Res. 1974 Feb;1(2):217-21. doi: 10.1093/nar/1.2.217.

DOI:10.1093/nar/1.2.217

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343340/

Abstract

Complementary and antiparallel oligonucleotides bind to exposed regions of the tRNA molecule. Aminoacylation in the presence of triplets has been used to determine the role of the anticodon in the interaction between methionyl-tRNA synthetase and initiator tRNA. ApUpG has no effect on the charging even when 70% of the tRNA is bound to the triplet, whereas in the presence of GpGpU which binds to the A-C-C sequence adjacent to the 3' terminal adenosine that fraction of the tRNA which is bound to the triplet is completely unavailable for charging. Hence the anticodon is probably not involved in a primary interaction while the A-C-C-A-OH clearly is. This conclusion is supported by the failure of the isolated anticodon loop and stem oligonucleotides to inhibit the aminoacylation reaction.

摘要

互补且反平行的寡核苷酸与tRNA分子的暴露区域结合。在三联体存在的情况下进行氨酰化反应，已被用于确定反密码子在甲硫氨酰-tRNA合成酶与起始tRNA相互作用中的作用。即使70%的tRNA与三联体结合，ApUpG对氨酰化反应也没有影响，而在与3'末端腺苷相邻的A-C-C序列结合的GpGpU存在时，与三联体结合的那部分tRNA完全无法进行氨酰化反应。因此，反密码子可能不参与主要相互作用，而A-C-C-A-OH显然参与其中。分离的反密码子环和茎寡核苷酸不能抑制氨酰化反应，这一结论得到了该结果的支持。

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本文引用的文献

1

Complementary oligonucleotide binding to the anticodon loop of fMet-transfer RNA.与甲酰甲硫氨酸转运RNA反密码子环结合的互补寡核苷酸。

Nature. 1970 Feb 7;225(5232):508-10. doi: 10.1038/225508a0.

2

Chemical studies on methionyl-tRNA synthetase from Escherichia coli.大肠杆菌甲硫氨酰 - tRNA合成酶的化学研究。

J Mol Biol. 1970 Sep 14;52(2):165-78. doi: 10.1016/0022-2836(70)90023-9.

3

The stability of a codon transfer RNA complex.密码子转运RNA复合体的稳定性。

Eur J Biochem. 1970 Feb;12(3):527-32. doi: 10.1111/j.1432-1033.1970.tb00882.x.

4

Studies on the Escherichia coli Methionine transfer ribonucleic acids.大肠杆菌甲硫氨酸转移核糖核酸的研究。

Eur J Biochem. 1969 Mar;8(1):93-100. doi: 10.1111/j.1432-1033.1969.tb00500.x.

5

The mechanism of action of methionyl-tRNA synthetase from Escherichia coli. 1. Fluorescence studies on tRNAMet binding as a function of ligands, ions and pH.来自大肠杆菌的甲硫氨酰 - tRNA合成酶的作用机制。1. 作为配体、离子和pH函数的tRNAMet结合的荧光研究。

Eur J Biochem. 1973 Jul 2;36(1):213-26. doi: 10.1111/j.1432-1033.1973.tb02903.x.

6

Evidence of "three point" attachment of tRNA to methionyl tRNA synthetase.tRNA与甲硫氨酰tRNA合成酶“三点”结合的证据。

Nat New Biol. 1973 May 23;243(125):103-5.

7

Loss of methionine acceptor activity resulting from a base change in the anticodon of Escherichia coli formylmethionine transfer ribonucleic acid.由于大肠杆菌甲酰甲硫氨酸转移核糖核酸反密码子中的碱基变化导致甲硫氨酸受体活性丧失。

J Biol Chem. 1973 Feb 25;248(4):1341-5.

8

Complementary oligonucleotide binding to transfer RNA.

J Mol Biol. 1972 Mar 14;65(1):25-41. doi: 10.1016/0022-2836(72)90489-5.

9

Codon-anticodon interaction of methionine specific tRNAs.

Biochem Biophys Res Commun. 1972 Mar 24;46(6):2100-6. doi: 10.1016/0006-291x(72)90765-6.

10

Three-dimensional structure of tRNA.转运核糖核酸的三维结构

Prog Nucleic Acid Res Mol Biol. 1971;11:391-421. doi: 10.1016/s0079-6603(08)60333-5.

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