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[百合花药组织在小孢子发生和配子发生过程中DNA和组蛋白的细胞化学研究]

[Cytochemical study of the DNA and histone in the tissues of the lily anther in the course of microsporo- and gametogenesis].

作者信息

Reznikova S A, Bugara A M, Erokhina A I

出版信息

Ontogenez. 1979;10(3):276-84.

PMID:460798
Abstract

The DNA and histone content in the microsporocytes, microspores, generative and vegetative cells of the pollen grain of Lilium candidum L., as well as in the anther wall tissues, was estimated by double wavelength cytophotometry. The lack of histone, as compared with DNA content, was demonstrated in the microsporocytes at the late premeiotic interphase and early meiotic prophase, as well as in the young microspores and anther wall tissues. The analysis of hydrolysis curves suggests the increase of non-condensed chromatin during endothelial cell differentiation.

摘要

通过双波长细胞光度法估算了麝香百合花粉粒的小孢子母细胞、小孢子、生殖细胞和营养细胞以及花药壁组织中的DNA和组蛋白含量。与DNA含量相比,在减数分裂前间期后期和减数分裂前期早期的小孢子母细胞以及幼嫩小孢子和花药壁组织中均显示出组蛋白缺乏。水解曲线分析表明,在内皮细胞分化过程中,非浓缩染色质增加。

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