Reznikova S A, Erokhina A I
Tsitol Genet. 1980 May-Jun;14(3):31-6.
Double-wavelength cytophotometry of the Feulgen-stained nuclei was used to detect the different ratio of condensed and non-condensed chromatin in the anther tissues in the course of their differentiation on the basis of hydrolysis curves in IN HCl at 60 degrees C. Formaline added to the fixating mixture diminishes the DNA losses during hydrolysis in all types of cells under study. Meanwhile, its effect is manifested depending on physiocochemical state of chromatin.
采用福尔根染色细胞核的双波长细胞光度法,根据60℃下1N盐酸中的水解曲线,检测花药组织在分化过程中浓缩染色质与非浓缩染色质的不同比例。添加到固定混合物中的甲醛减少了所有研究类型细胞水解过程中的DNA损失。同时,其效果取决于染色质的物理化学状态。