Geiman J M, Christensen J R, Drexler H
J Virol. 1974 Dec;14(6):1430-4. doi: 10.1128/JVI.14.6.1430-1434.1974.
Bacteria containing phage lambda in the vegetative state were produced either by induction of lambda lysogens or by infection of sensitive cells with lambda. These cells were superinfected with T1, and assayed for the production of lambda, T1, or both. Although most of the cells produced only lambda or T1, approximately 10% of the infectious centers were dual yielders. Examination of the progeny phage produced by the population of mixedly-infected cells showed that there was little, if any, phenotypic mixing, as determined by adsorption phenotype. T1am mutants in a variety of T1 genes were tested for their ability to exclude lambda, but none were defective in this ability. One gene of T1, gene 4, can be complemented by lambda.
通过诱导λ溶原菌或用λ感染敏感细胞来产生处于营养状态的含有噬菌体λ的细菌。这些细胞用T1进行超感染,并检测λ、T1或两者的产生情况。虽然大多数细胞只产生λ或T1,但约10%的感染中心是双产物产生者。对混合感染细胞群体产生的子代噬菌体进行检查发现,根据吸附表型确定,几乎没有(如果有的话)表型混合现象。对各种T1基因中的T1am突变体进行了排除λ能力的测试,但没有一个在这种能力上有缺陷。T1的一个基因,即基因4,可以被λ互补。