Barta K, Zissler J
J Virol. 1974 Dec;14(6):1451-7. doi: 10.1128/JVI.14.6.1451-1457.1974.
We have studied the effect of delta mutations in phage lambda on DNA synthesis as assayed by the accumulation of lambda DNA in infected cells. We find that delta mutants appear to generate somewhat less DNA than lambda(+) in a rec(+) host, suggesting the wild-type delta gene may act in DNA replication. An additional clue to delta function arises if replication is measured in the gamma-negative situation where concatemer formation is abortive. In this situation, the wild-type delta gene has an "inhibitory" effect on replication. A similar inhibitory effect on replication due to delta is observed after infection of P(2) lysogens. We conclude from these studies that the delta gene may act with alpha, beta, and gamma genes, possibly in a process affecting DNA replication.
我们研究了噬菌体λ中的δ突变对DNA合成的影响，该影响通过λDNA在感染细胞中的积累来测定。我们发现，在rec(+)宿主中，δ突变体产生的DNA似乎比λ(+)略少，这表明野生型δ基因可能在DNA复制中起作用。如果在γ阴性情况下测量复制，其中串联体形成失败，就会出现关于δ功能的另一个线索。在这种情况下，野生型δ基因对复制有“抑制”作用。在感染P(2)溶原菌后，观察到δ对复制有类似的抑制作用。我们从这些研究中得出结论，δ基因可能与α、β和γ基因一起起作用，可能在一个影响DNA复制的过程中。
