遗传重组在噬菌体λ DNA复制中的作用。I. δ基因的遗传学特征
Role of genetic recombination in DNA replication of bacteriophage lambda. I. Genetic characterization of the delta gene.
作者信息
Barta K, Tavernier P, Zissler J
出版信息
J Virol. 1974 Dec;14(6):1445-50. doi: 10.1128/JVI.14.6.1445-1450.1974.
Abstract
We describe the isolation and genetic characterization of point mutations in gene delta, including a temperature-sensitive mutation (del(206)). Genetic methods enable the extraction of a delta mutation from the triple mutant (del,red,gam) and the construction of new genotypes, including del,red and del,gam double mutants. Tests of plating efficiency indicate gene delta is essential for normal phase growth on the polA host. The possible association of delta in a system involving alpha, beta, and gamma is considered.
摘要
我们描述了基因δ中位点突变的分离及遗传特征，包括一个温度敏感突变（del(206)）。遗传方法能够从三重突变体（del,red,gam）中提取δ突变，并构建新的基因型，包括del,red和del,gam双突变体。平板效率测试表明，基因δ对于在polA宿主上的正常阶段生长至关重要。我们还考虑了δ在涉及α、β和γ的系统中的可能关联。
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引用本文的文献
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Role of genetic recombination in DNA replication of bacteriophage lambda. II. Effect in DNA replication by gene delta.基因重组在噬菌体λ DNA复制中的作用。II. δ基因对DNA复制的影响。
J Virol. 1974 Dec;14(6):1451-7. doi: 10.1128/JVI.14.6.1451-1457.1974.
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