P2噬菌体与大肠杆菌dnaB基因的相互作用。
Interaction of P2 bacteriophage with the dnaB gene of Escherichia coli.
作者信息
Sunshine M, Usher D, Calendar R
出版信息
J Virol. 1975 Aug;16(2):284-9. doi: 10.1128/JVI.16.2.284-289.1975.
Abstract
The dnaB gene product of Escherischia coli is required for multiplication of temperate phage P2. At 37 C in dnaB-ts mutnats, P2 will not plaque and gives a very small burst of progeny. P2 mutants have been isolated which can grow well enough to plaque under these conditions. This type of phage mutant is cis dominant, and one such mutant (P2rlb1) has been mapped near the left end of the early gene B and to the right of the cox4 (excision) mutation. The rlb1 mutation does not lie at the replication origin, but may affect transcription in the early region, which includes the replication origin. It may also represent a site on the P2 DNA which interacts with the dnaB gene product.
摘要
大肠杆菌的dnaB基因产物是温和噬菌体P2增殖所必需的。在dnaB温度敏感突变体中，于37℃时，P2不能形成噬菌斑，且子代噬菌体的爆发量极少。已经分离出了在这些条件下能够生长良好并形成噬菌斑的P2突变体。这种类型的噬菌体突变体是顺式显性的，并且其中一个这样的突变体（P2rlb1）已被定位在早期基因B的左端附近且在cox4（切除）突变的右侧。rlb1突变并不位于复制起点，但可能影响早期区域（包括复制起点）的转录。它也可能代表P2 DNA上与dnaB基因产物相互作用的一个位点。
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