Preuss H G, Byrne D, Shim P S
J Pharmacol Exp Ther. 1976 Apr;197(1):199-205.
After para-aminohippurate (PAH) infusion into rats, urine pH decreased and urine ammonium excretion increased. Because augmented urine flow and decreased urine pH could not explain entirely the enhanced ammonium excretion, an increased ammonia production was postulated as a contributing influence. This was supported by the in vitro findings that PAH could increase slice ammoniagenesis from glutamine. The ability of PAH to stimulate ammoniagenesis in vitro was attributed to enhanced phosphate-dependent glutaminase activity. We conclude that PAH infusions at certain concentrations in vivo can alter ammonium excretion through increased renal ammonia production. The latter may be secondary to enhanced phosphate-dependent glutaminase activity.
给大鼠输注对氨基马尿酸（PAH）后，尿pH值降低，尿铵排泄增加。由于尿量增加和尿pH值降低并不能完全解释铵排泄增加的现象，因此推测氨生成增加是一个促成因素。体外研究结果支持了这一点，即PAH可增加切片由谷氨酰胺生成氨的过程。PAH在体外刺激氨生成的能力归因于依赖磷酸盐的谷氨酰胺酶活性增强。我们得出结论，体内特定浓度的PAH输注可通过增加肾脏氨生成来改变铵排泄。后者可能继发于依赖磷酸盐的谷氨酰胺酶活性增强。
