Onderdonk A B, Weinstein W M, Sullivan N M, Bartlett J G, Gorbach S L
Infect Immun. 1974 Dec;10(6):1256-9. doi: 10.1128/iai.10.6.1256-1259.1974.
An animal model simulating intra-abdominal sepsis was produced by implanting large bowel contents into the pelvic region of rats. Bacteriological analysis of infected sites showed quantitative differences according to the stage of disease. During the initial, often lethal, peritonitis stage, Escherichia coli (mean concentration, 10(6)/ml), enterococci (10(5)) and Bacterioides fragilis (10(6)) were always present. Blood cultures obtained during this phase were uniformly positive, with E. coli being the principal isolate. Animals that survived this early acute peritonitis stage developed indolent intra-abdominal abscesses. The major isolates in abscess contents were B. fragilis (10(8.7)) and Fusobacterium (10(8.6)); E. coli (10(7.8)) and enterococci (10(5.7)) were also present but in lesser concentrations. Rank order analysis of these four species in peritoneal exudates and abscess pus showed that the two aerobes outranked the two anaerobes during the early stage of the disease, whereas the reverse was true in abscesses. These experiments also illustrated that a major simplification of the original fecal inoculum occurred, even though the subsequent infection remained bacteriologically complex.
通过将大肠内容物植入大鼠盆腔区域建立了模拟腹腔内脓毒症的动物模型。对感染部位的细菌学分析显示，根据疾病阶段存在数量差异。在最初的、通常致命的腹膜炎阶段，始终存在大肠杆菌（平均浓度为10⁶/ml）、肠球菌（10⁵）和脆弱拟杆菌（10⁶）。在此阶段获得的血培养结果均为阳性，主要分离菌为大肠杆菌。在早期急性腹膜炎阶段存活下来的动物发展为慢性腹腔内脓肿。脓肿内容物中的主要分离菌为脆弱拟杆菌（10⁸·⁷）和梭杆菌（10⁸·⁶）；也存在大肠杆菌（10⁷·⁸）和肠球菌（10⁵·⁷），但浓度较低。对这四种菌在腹腔渗出液和脓肿脓液中的排序分析表明，在疾病早期，两种需氧菌的数量超过两种厌氧菌，而在脓肿中情况则相反。这些实验还表明，尽管随后的感染在细菌学上仍然复杂，但原始粪便接种物发生了重大简化。
