Moore W E, Holdeman L V
Appl Microbiol. 1974 May;27(5):961-79. doi: 10.1128/am.27.5.961-979.1974.
Quantitative and qualitative examination of the fecal flora of 20 clinically healthy Japanese-Hawaiian males was carried out by using anaerobic tube culture techniques. Cultural counts were 93% of the microscopic clump counts. Isolated colonies were selected in a randomized manner to give an unbiased sampling of the viable bacterial types. Each isolate was characterized for species identification. From a total of 1,147 isolates, 113 distinct types of organisms were observed. Statistical estimates indicate that these types account for 94% of the viable cells in the feces. The quantitative composition of the flora of this group of people, together with differential characteristics of previously unreported species, is presented for those kinds of bacteria which each represented at least 0.05% of the flora.
采用厌氧管培养技术,对20名临床健康的日裔夏威夷男性的粪便菌群进行了定量和定性检测。培养计数为显微镜下菌团计数的93%。以随机方式选择分离菌落,以便对活菌类型进行无偏抽样。对每个分离株进行物种鉴定。在总共1147个分离株中,观察到113种不同类型的微生物。统计估计表明,这些类型占粪便中活菌的94%。本文给出了这群人菌群的定量组成,以及此前未报道物种的鉴别特征,这些细菌每种至少占菌群的0.05%。