Kindle K L, Firtel R A
Nucleic Acids Res. 1979 Jun 11;6(7):2403-22. doi: 10.1093/nar/6.7.2403.
Two recombinant plasmids, M4 and KH10, carrying Dictyostelium DNA inserted into the Eco RI restriction endonuclease site of pMB9 by poly(dA)-poly(dT) tailing, were selected for study because they are complementary to abundant mRNA populations from Dictyostelium. Both plasmids have been shown to hybridize a heterogeneous size class of mRNAs which, in the case of KH10, comprise 5-10% of the pulse-labeled poly(A)+ RNA from vegetative cells. Analysis of the sequence organization of the two pieces of Dictyostelium DNA shows that they consist mostly of single-copy sequences with a short DNA sequence which is repeated in the genome and interspersed with single-copy DNA. These and other results suggest that the majority of the hybridization of pulse-labeled mRNA to M4 and KH10 is to the short "repeated" DNA sequences. In the genome, members of these repeat families appear to be transcribed onto a population of different single-copy mRNAs. Additional results show that M4 DNA contains a sequence which is entirely complementary to a discrete mRNA.
通过聚（dA）-聚（dT）加尾将携带盘基网柄菌DNA的两个重组质粒M4和KH10插入pMB9的Eco RI限制性内切酶位点，由于它们与盘基网柄菌丰富的mRNA群体互补，因此被选来进行研究。已证明这两个质粒都能与一类大小不均一的mRNA杂交，就KH10而言，这类mRNA占营养细胞脉冲标记的聚腺苷酸加尾RNA的5%-10%。对这两段盘基网柄菌DNA的序列组织分析表明，它们主要由单拷贝序列组成，有一段短的DNA序列在基因组中重复，并散布于单拷贝DNA之间。这些结果以及其他结果表明，脉冲标记的mRNA与M4和KH10的杂交大多是与短的“重复”DNA序列发生。在基因组中，这些重复家族的成员似乎被转录到一群不同的单拷贝mRNA上。另外的结果表明，M4 DNA含有一个与一种离散mRNA完全互补的序列。
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